CFX96TOUCH Fluorescent Quantitative PCR meafaigaluega

Ole CFX96Touch fluorescent quantitative PCR e mafai ona fa'aoga ile tele o su'esu'ega o le nucleic acid quantification, gene expression level analysis, gene mutation detection, GMO detection, ma le auiliiliga o oloa.

Siosiomaga Galuega:
1.1 Galulue vevela: 5-31 ° C
1.2 Galulue ma susū: susū fa'atatau ≤80%
1.3 Malosiaga galue: 100-240 VAC, 50-60Hz
Fa'atinoga ma mana'oga fa'apitoa ole CFX96Touch fluorescence quantitative PCR
3.1 Fa'atinoga autu (* o le fa'ailoga e tatau ona fa'amalieina)
* 3.1.1 E ono auala o suʻega, 5% PCR e mafai ona iloa, ma e 5 faʻasologa o kenera e mafai ona iloa i le taimi e tasi, ma o le auala faʻapitoa e iloa ai le FRET e maua i le taimi e tasi.
* 3.1.2 Faatasi ai ma le malosi o le vevela gradient PCR galuega, e mafai ona e tamoe 8 vevela eseese i le taimi e tasi, taʻitasi vevela incubation.
3.1.3 Tatala o reagents atoatoa, su'esu'ega eseese ma fa'afofoga fa'afoma'i fa'aaoga;
3.1.4 Talafeagai mo le tele o auala fluorescence e pei o Taqman, Molecular Beacon, Fret probe, Sybr Green i, ma isi;
3.1.5 Tatala, 0.2ml paipa tasi, octal, 96-well ipu, ma isi.
* 3.1.6 e mafai ona tamoʻe tutoʻatasi, faʻaoga moni tuusao, e leai se manaʻoga e faʻafesoʻotaʻi le komepiuta e mataʻituina le PCR fluorescent amplification curve i le taimi moni;
3.2 Manaoga faʻapitoa faʻapitoa (* o le faʻailoga e tatau ona ausia)
* 3.2.1 Faʻataʻitaʻiga gafatia: 96 × 0.2ml, faʻamatalaga masani 96-well plates (12 × 8) e mafai ona faʻaaogaina;
3.2.2 Sapalai Ituaiga: 0.2ml paipa tasi, valu so'otaga, 96-vai ipu, ma isi.
3.2.3 Reaction System: 1-50μL (fautuaina 10-25 μL);
* 3.2.4 Punavai malamalama: ono LED ma faamama;
* 3.2.5 Su'esu'e: ono diodes photosensitive ma faamama;
* 3.2.6 lita malulu saosaoa: 5 ° C / sec;
3.2.7 Fa'atonuga o le vevela: 0 -100 ° C;
3.2.8 Sa'o o le vevela: ± 0.2 ° C (90 ˚C);
3.2.9 Tulaga tutusa o le vevela: ± 0.4 ° C (90 ˚C i totonu ole 10 sekone);
* 3.2.10 Galulue Fa'alili ole Temperature: Tafe 8 vevela eseese ile taimi e tasi; fa'asologa o le vevela fa'asolosolo: 30 -100 ° C; fa'alili ole vevela va'aiga eseese: 1 - 24 ° C; gradient vevela taimi incubation: tutusa;
3.2.11 fa'afiafiaga / fa'avevela fa'alava umi galu: 450-730 nm;
3.2.12 Ma'ale'ale: E mafai ona maua se kopi kopi tasi i le genome o le tagata;
3.2.13 Fa'asologa fa'avavevave: 10 aofa'i;
3.2.14 Fa'aaliga: 8.5 inisi lanu pa'i mata;
3.2.15 Faʻamatalaga Faʻamatalaga Faʻamatalaga: Aofaʻi Faʻasologa Faʻasologa, Faʻalili Meli, CT poʻo le ΔΔCT Gene Expression Analysis, Suʻesuʻega Faʻasologa o Genodes Tele ma Faʻateleina Faʻatatauga Faʻatatau, Faʻamatalaga Faʻamatalaga Faʻamatalaga Faʻamatalaga Faʻamatalaga Faʻamatalaga, Allelic Analysis, Type Analysis, Have Gene, faʻafefe faʻafefe o galuega ;
3.2.16 Fa'asalalau Fa'amatalaga: Excel, Word, po'o le PowerPoint. O le lipoti a le tagata fa'aoga o lo'o i ai fa'asologa o ta'avale, fa'ata'ita'iga, ma taunu'uga o fa'amaumauga o laulau, lea e mafai ona lolomi pe fa'asaoina e avea ma PDF;
* 3.2.17 Chromosomal structure suʻesuʻega: O se metotia o suʻesuʻega faʻatusatusa o fausaga chromatin e ala i le faʻatusatusaina o le faʻaleagaina o le DNA genomic e ala i le faʻatusatusaina o le DNA genomic. E faʻamaonia moni le faʻamaopoopoina o le maualuga i le va o le fausaga o le chromatin ma le faʻaaliga o gene;